primary renal epithelial cells  (ATCC)

Journal: bioRxiv

Article Title: Site-Dependent Decoupling of Drug-Biomarker Associations in Clear Cell Renal Cell Carcinoma Revealed by Functional Profiling of Patient-Derived Cell Models

doi: 10.64898/2026.04.23.720088

Figure Lengend Snippet: A. Drug sensitivity scores of representative PDC models for the drugs exhibiting mean DSS > 10 in at least one sample. Tumor cell fraction estimates, drug plate, passage, sample type, site and either presence or absence of commonly observed ccRCC mutations are indicated at the top of the heatmap while the functional drug class of each drug is indicated on the left. All DSRT replicates were averaged (mean) per matching WES sample (including replicates screened in 2D/3D conditions with FS2A (116 drugs)/FO5A (528 drugs) plates. For P1, P15 and P24, overall lower DSS values were observed in 3D-than 2D-DSRT). B. Intra-patient variability in drug sensitivity profiles of samples derived from T, VC, and MET for patients P1 and P10. C. Median DSS across T, MET, VC, cell line, B, and control (primary kidney epithelial cells) samples and functional drug classes.

Article Snippet: Cortical (PCS-400-011, ATCC) and proximal (PCS-400-010, ATCC) primary renal epithelial cells, together with ACHN (CRL-1611, ATCC), A-498 (ACC 55, DSMZ), Caki-1 (ACC 731, DSMZ), Caki-2 (ACC 54, DSMZ), and 786-O (CRL-1932, ATCC) cell lines, were cultured and maintained in accordance with the manufacturers’ instructions.

Techniques: Functional Assay, Derivative Assay, Control

Journal: Open Forum Infectious Diseases

Article Title: Induction of Human β-Defensin-2 by Vaginal Lactobacillus crispatus Strains in Vaginal Epithelial Cells Correlates With Their Adhesion Abilities

doi: 10.1093/ofid/ofag193

Figure Lengend Snippet: Ability of Lactobacillus crispatus and Lactobacillus gasseri to enhance human β-defensin-2 ( HβD2 ) mRNA expression was associated with its binding capacity to vaginal epithelial cells. L. crispatus or L. gasseri was incubated with VK2/E6E7 at a multiplicity of infection (MOI) of 50 for ( A ) 4 or ( B ) 6 h. A , Nuclei of both bacterial and host cells were stained blue with DAPI. White scale bar represents 10 μm. Microscopic bacterial cell counts were performed on nine images per sample. B , A correlation is highlighted between induced HβD2 mRNA expression and the number of L. crispatus strains that adhere to vaginal epithelial cells. Dotted lines show the 95% confidence bands of the best-fit lines. All experiments were conducted in at least three independent trials. Each dot indicates the mean values. The correlation was evaluated using Spearman's correlation coefficient; **** indicates statistically significant differences ( P < .0001).

Article Snippet: VK2/E6E7 (ATCC CRL-2616), primary immortalized human vaginal epithelial cells, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Binding Assay, Incubation, Infection, Staining

Journal: Open Forum Infectious Diseases

Article Title: Induction of Human β-Defensin-2 by Vaginal Lactobacillus crispatus Strains in Vaginal Epithelial Cells Correlates With Their Adhesion Abilities

doi: 10.1093/ofid/ofag193

Figure Lengend Snippet: Lactobacillus crispatus -induced human β-defensin-2 ( HβD2 ) mRNA expression in cervical epithelial cells, but not in endometrial epithelial cells. Heat-killed L. crispatus was incubated with ( A ) A2EN and ( B ) HEC-1A cells at a multiplicity of infection (MOI) of 50 for 6 h. After extracting RNA, cDNA was prepared, and quantitative PCR was performed to measure the mRNA levels for HβD2 or GAPDH . Each value was normalized to GAPDH mRNA levels. Normalized value in Dulbecco's phosphate-buffered saline (DPBS)-treated cells was set to 1. Vertical axis indicates the relative changes compared with the DPBS control of HβD2 mRNA. All experiments were conducted in at least four independent trials. Vertical bars represent mean ± standard deviations. Statistically significant difference (*; P < .05) is presented based on unpaired t -test results (A: DPBS vs HMS-115 Heat-killed; P = .0105).

Article Snippet: VK2/E6E7 (ATCC CRL-2616), primary immortalized human vaginal epithelial cells, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Incubation, Infection, Real-time Polymerase Chain Reaction, Saline, Control

Journal: Open Forum Infectious Diseases

Article Title: Induction of Human β-Defensin-2 by Vaginal Lactobacillus crispatus Strains in Vaginal Epithelial Cells Correlates With Their Adhesion Abilities

doi: 10.1093/ofid/ofag193

Figure Lengend Snippet: Impact of Lactobacillus crispatus bacterial viability on vaginal epithelial cells. L. crispatus was incubated with VK2/E6E7 cells at a multiplicity of infection (MOI) of 50 for 6 h. After extracting RNA from VK2/E6E7 cells, cDNA was prepared, and quantitative PCR was performed to measure mRNA levels for IL8 ( A ), TNFα ( B ), and MUC1 ( C ). Each value was normalized to GAPDH mRNA levels, with the value in Dulbecco's phosphate-buffered saline (DPBS)-treated cells set to 1. Vertical axis represents the relative changes for each target mRNA compared with the DPBS control, while the horizontal axis shows the live or heat-killed L. crispatus HMS-115. All experiments were conducted in three independent trials. Vertical bars represent mean ± standard deviations. Statistically significant differences (**; P < .01, ***; P < .001, ****; P < .0001) are presented based on one-way analysis of variance followed by Tukey's multiple comparison test (A: DPBS vs HMS-115 Live; P = .0002, HMS-115 Live vs Heat-killed; P < .0001, B: DPBS vs HMS-115 Live; P < .0001, HMS-115 Live vs Heat-killed; P < .0001, C: DPBS vs HMS-115 Heat-killed; P = .0034, HMS-115 Live vs Heat-killed; P = .0061).

Article Snippet: VK2/E6E7 (ATCC CRL-2616), primary immortalized human vaginal epithelial cells, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Incubation, Infection, Real-time Polymerase Chain Reaction, Saline, Control, Comparison

Journal: Open Forum Infectious Diseases

Article Title: Induction of Human β-Defensin-2 by Vaginal Lactobacillus crispatus Strains in Vaginal Epithelial Cells Correlates With Their Adhesion Abilities

doi: 10.1093/ofid/ofag193

Figure Lengend Snippet: Schematic representation of the interaction between vaginal Lactobacillus crispatus and human β-defensin-2 (HβD-2) production. HβD-2 mRNA expression and production in vaginal epithelial cells varied significantly across L. crispatus strains. Vaginal L. crispatus with strong adhesion capability increased HβD-2 production in vaginal epithelial cells.

Article Snippet: VK2/E6E7 (ATCC CRL-2616), primary immortalized human vaginal epithelial cells, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing